Figure 6
Evidence that B. anthracis 7702 has a high affinity and specificity manganese transporter involved in arginase activity. B. anthracis 7702 were grown in BHI broth in the absence or presence of metal (CoCl2, MnSO4, NiCl2) at various concentrations. No effect on growth was observed at the concentrations used. Cells were harvested and assayed directly (viable whole cells) without heat treatment (ice, 30 min) at pH 9.0 using BTP-buffered arginine. Inset. B. anthracis grown with 0.2 to 1.0 μM MnSO4.