Figure 1

B. anthracis 7702 extracts show optimal catalytic activity with nickel. Cells were grown on CBA plates at 37°C for 24 h in 5% CO₂ in air, harvested, and extracts prepared as described in Methods. The extracts were assayed with 5 mM (final concentration) of the appropriate metal after heat activation at pH 6.3 (A) or 9.0 (B) (Bis Tris Propane [BTP]-buffered arginine).