Table 1 Typical results of DSN purification from crab hepatopancreas.
Stage of purification | Total protein, mg | Specific activity, Kunitz/mg | Activity yield, % | Fold purification |
|---|---|---|---|---|
Kamchatka crab hepatopancreas extract | 1948.0 | 51 | 100 | 1.0 |
Chromatography on DEAE MacroPrep | 275.2 | 265 | 73 | 5.2 |
Chromatography on Phenyl Sepharose | 70.0 | 801 | 56 | 15.7 |
Re-chromatography on Phenyl Sepharose | 10.0 | 4900 | 49 | 96.1 |
Chromatography on Hydroxyapatite | 8.1 | 5200 | 43 | 102.0 |
Chromatography on Heparin Sepharose | 1.4 | 29000 | 40 | 568.6 |
Gel filtration on Sephacryl S-200 | 0.95 | 31500 | 30 | 617.7 |