Figure 7From: Isolation, characterization and molecular cloning of Duplex-Specific Nuclease from the hepatopancreas of the Kamchatka crabDSN activity against ds and ss RNA. Agarose gel electrophoresis of ds or ss RNA and ds DNA before and after treatment with DSN. Lanes: 1, untreated lambda phage DNA (500 ng) and total RNA (50 ng) from cultured cells; 2, lambda phage DNA and total RNA after incubation with 2 units of DSN for 30 min at 37°C; 3, untreated lambda phage DNA and synthetic ds RNA (2 μg); 4, lambda phage DNA and synthetic ds RNA after incubation with 2 units of DSN for 30 min at 37°C; m, 1-kb ladder (SibEnzyme).Back to article page