Figure 3

A: Relationship between divalent cation concentration and DSN activity. The incubation mixtures (50 mM Tris-HCl, pH 7.15) contained calf thymus DNA (50 μg/ml), 8 units/ml DSN and different concentrations of MnCl₂, CoCl₂, MgCl₂, CdCl₂, CaCl₂. B: Relationship between DSN activity and concentration of magnesium chloride, calcium chloride and their mixture. The incubation mixtures (50 mM Tris-HCl, pH 7.15) contained calf thymus DNA (50 μg/ml), 8 units/ml DSN and different concentrations of MgCl₂, CaCl₂, and mixtures of MgCl₂ and CaCl₂.