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Figure 2 | BMC Biochemistry

Figure 2

From: Isolation, characterization and molecular cloning of Duplex-Specific Nuclease from the hepatopancreas of the Kamchatka crab

Figure 2

Purification of DSN from the crab hepatopancreas. A: Composition of protein samples obtained at different stages of DSN purification, determined by SDS-PAGE and Coomassie R-250 staining. B: Composition of protein samples obtained at different stages of DSN purification, determined by SDS-PAGE and immunoblotting.Lanes: 1, crude extract; 2, fractions obtained after DEAE MacroPrep chromatography; 3 and 4, combined fractions after first and second Phenyl Sepharose chromatography steps; 5, fractions obtained after hydroxyapatite chromatography; 6, fractions obtained after Heparin Sepharose chromatography; 7, final preparation obtained after Sephacryl S200 chromatography. M, molecular mass marker with molecular mass (in kDa) indicated at right.

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