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Figure 7 | BMC Biochemistry

Figure 7

From: The Mycobacterium tuberculosis Rv2540c DNA sequence encodes a bifunctional chorismate synthase

Figure 7

Primary and multiple deuterium kinetic isotope effects. Measurements of primary deuterium kinetic isotope effects for Mt CS yielded values of 3.5 ± 0.2 and 3.0 ± 0.4 for, respectively, DV and DV/K. Inset: Measurements of primary isotope effect in 90 atom % D2O (multiple) yielded values of 1.3 ± 0.1 and 2.2 ± 0.1 for D2OV[4S-2H]NADHand D2OV/K[4S-2H]NADH, respectively. In both [4S-1H]NADH () or [4S-2H]NADH (■) is varied in the reaction mixture. L represents either [4S-1H]NADH or [4S-2H]NADH.

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