Figure 2

Recombinant Mt CS protein expression and purification. (A) Analysis of Mt CS expression using E. coli Rosetta(DE3) host cells by SDS-poliacrilamide gel 12%. Lanes: 1 – pellet Rosetta(DE3)+pET23a(+)::aroF; 2 – soluble extract Rosetta(DE3)+pET23a(+)::aroF; 3 – pellet Rosetta(DE3)+pET23a(+) (negative control); 4 – soluble extract Rosetta(DE3)+pET23a(+) (negative control); 5 – protein molecular weight standards, high range (Gibco) (43 kDa; 29 kDa; 18 kDa; 14 kDa) (B) Analysis of purification steps of Mt CS by SDS-polyacrylamide gel 12%. Lanes: 1 – soluble extract after dialysis; 2 – soluble extract after Q Sepharose Fast Flow column; 3 – CS after Phenyl-Sepharose column; 4 – CS after MonoQ column; 5 – protein molecular weight standards, high range (Gibco) (200 kDa; 97 kDa; 68 kDa; 43 kDa; 29 kDa; 18 kDa; 14 kDa).