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Table 6 Effects of assay temperature and glucose on the S0.5 AMP for bound AMPD from skeletal muscle of control versus frozen wood frogs.

From: Regulation of 5'-adenosine monophosphate deaminase in the freeze tolerant wood frog, Rana sylvatica

 

S0.5 AMP (mM)

 

Control

Frozen

25°C, no glucose

0.46 ± 0.03

1.50 ± 0.19

25°C, 250 mM glucose

0.30 ± 0.04 a

0.24 ± 0.03 a

5°C, no glucose

0.70 ± 0.03 a

1.77 ± 0.03 a

5°C, 250 mM glucose

0.87 ± 0.04 a

2.14 ± 0.04 a

  1. Data are means ± SEM, for determinations on Sephadex G50-filtered muscle extracts from n = 4 individual frogs. a – Significantly different from the corresponding value at 25°C without glucose as assessed by the Student's t-test, P < 0.05.
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BMC Biochemistry

ISSN: 1471-2091

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