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Table 5 Inhibitor effects (I50 values) on AMPD from skeletal muscle of control versus frozen wood frogs

From: Regulation of 5'-adenosine monophosphate deaminase in the freeze tolerant wood frog, Rana sylvatica

 

Control

Frozen

 

Free AMPD

Bound AMPD

Free AMPD

Bound AMPD

Mg·GTP, mM

0.15 ± 0.02

0.08 ± 0.01

0.08 ± 0.01 a

0.10 ± 0.01

IMP, mM

NE

NE

NE

0.23 ± 0.02 a

KCl, mM

660 ± 44

148 ± 34

702 ± 21 a

162 ± 24

NaCl, mM

973 ± 20

141 ± 31

824 ± 26 a

133 ± 21

NH4Cl, mM

276 ± 5.0

ND

254 ± 10

ND

  1. Data are means ± SEM, for determinations on Sephadex G50-filtered muscle extracts from n = 4 individual frogs. I50 is the inhibitor concentration that reduces enzyme velocity by 50%. a – Significantly different from the corresponding value for muscle from control frogs as assessed by the Student's t-test, P < 0.05. NE, no effect. ND, not determined. I50 values were measured at an AMP concentration of 0.5 mM.
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BMC Biochemistry

ISSN: 1471-2091

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