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Table 4 Adenylate activation of wood frog skeletal muscle AMPD from control versus frozen frogs

From: Regulation of 5'-adenosine monophosphate deaminase in the freeze tolerant wood frog, Rana sylvatica

 

Control

Frozen

 

Free AMPD

Bound AMPD

Free AMPD

Bound AMPD

K a Mg·ATP, mM

1.90 ± 0.03

NE

0.63 ± 0.04 a

NE

Mg·ATP fold-activation

1.53 ± 0.09

NE

2.69 ± 0.20 a

NE

K a Mg·ADP, mM

1.12 ± 0.15 b

3.75 ± 0.37

0.54 ± 0.02 ab

1.14 ± 0.10 a

Mg·ADP fold-activation

2.17 ± 0.29

1.25 ± 0.21

5.01 ± 0.69 a

3.42 ± 0.24 a

  1. Data are means ± SEM, for determinations on Sephadex G50-filtered muscle extracts from n = 4 individual frogs. Significantly different from a the corresponding value in control frogs, b the corresponding value for bound AMPD in control frogs, by the Student's t-test, P < 0.05. NE, no effect. K a is the activator concentration that produces half-maximal activation; values were determined at 0.5 mM AMP. K a and fold activation values were calculated using a nonlinear least-squares regression kinetics program [39].
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BMC Biochemistry

ISSN: 1471-2091

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