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Table 3 Kinetic parameters for free AMPD before and after removal of low molecular weight ions and metabolites by centrifugation through Sephadex G50 columns

From: Regulation of 5'-adenosine monophosphate deaminase in the freeze tolerant wood frog, Rana sylvatica

  Before spun columns After spun columns
  S0.5 AMP (mM) n H S0.5 AMP (mM) n H
Control 2.16 ± 0.11 1.76 ± 0.06 1.35 ± 0.08a 2.37 ± 0.17 a
Frozen 2.19 ± 0.15 2.76 ± 0.10 a 1.68 ± 0.21 a 2.76 ± 0.27
  1. Data are means ± SEM, for determinations on muscle preparations from n = 4 individual frogs. a – Significantly different from the corresponding free control value prior to desalting by the Student's t-test, P < 0.05.