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Table 2 Kinetic parameters of AMPD in crude extracts of skeletal muscle from control (5°C-acclimated) and 24 h frozen wood frogs.

From: Regulation of 5'-adenosine monophosphate deaminase in the freeze tolerant wood frog, Rana sylvatica

 

S0.5 AMP (mM)

n H

Control

  

Free AMPD

2.16 ± 0.11 b

1.76 ± 0.06 bc

Bound AMPD

0.46 ± 0.03 ac

1.03 ± 0.07 ac

Frozen

  

Free AMPD

2.19 ± 0.15 b

2.76 ± 0.10 ab

Bound AMPD

1.50 ± 0.19 abc

1.12 ± 0.09 a

  1. Data are means ± SEM, for determinations on muscle preparations from n = 4 individual frogs S0.5 is the substrate concentration that produces half-maximal enzyme velocity; n H is the Hill coefficient. Significantly different from a the corresponding value for free AMPD from control frogs, b the corresponding value for bound AMPD from control frogs, or c the corresponding value for free AMPD from frozen frogs using the Student's t test, P < 0.05.