| S0.5 AMP (mM) | n
H
|
---|
Control | | |
Free AMPD | 2.16 ± 0.11 b | 1.76 ± 0.06 bc |
Bound AMPD | 0.46 ± 0.03 ac | 1.03 ± 0.07 ac |
Frozen | | |
Free AMPD | 2.19 ± 0.15 b | 2.76 ± 0.10 ab |
Bound AMPD | 1.50 ± 0.19 abc | 1.12 ± 0.09 a |
- Data are means ± SEM, for determinations on muscle preparations from n = 4 individual frogs S0.5 is the substrate concentration that produces half-maximal enzyme velocity; n
H
is the Hill coefficient. Significantly different from a the corresponding value for free AMPD from control frogs, b the corresponding value for bound AMPD from control frogs, or c the corresponding value for free AMPD from frozen frogs using the Student's t test, P < 0.05.