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Table 2 Kinetic parameters of AMPD in crude extracts of skeletal muscle from control (5°C-acclimated) and 24 h frozen wood frogs.

From: Regulation of 5'-adenosine monophosphate deaminase in the freeze tolerant wood frog, Rana sylvatica

  S0.5 AMP (mM) n H
Free AMPD 2.16 ± 0.11 b 1.76 ± 0.06 bc
Bound AMPD 0.46 ± 0.03 ac 1.03 ± 0.07 ac
Free AMPD 2.19 ± 0.15 b 2.76 ± 0.10 ab
Bound AMPD 1.50 ± 0.19 abc 1.12 ± 0.09 a
  1. Data are means ± SEM, for determinations on muscle preparations from n = 4 individual frogs S0.5 is the substrate concentration that produces half-maximal enzyme velocity; n H is the Hill coefficient. Significantly different from a the corresponding value for free AMPD from control frogs, b the corresponding value for bound AMPD from control frogs, or c the corresponding value for free AMPD from frozen frogs using the Student's t test, P < 0.05.