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Table 1 AMPD maximal activity (Vmax) and partitioning between free and bound forms in skeletal muscle from control (5°C-acclimated) and 24 h frozen frogs.

From: Regulation of 5'-adenosine monophosphate deaminase in the freeze tolerant wood frog, Rana sylvatica

 

Vmax (U/gww)

% of total activity

Control

  

Free AMPD

17.6 ± 1.53

79.7 ± 6.46

Bound AMPD

4.5 ± 0.03

20.3 ± 0.13

Total

22.1 ± 1.56

100.0

Frozen

  

Free AMPD

17.8 ± 0.69

64.6 ± 2.07 a

Bound AMPD

9.8 ± 0.03 a

35.4 ± 0.09 a

Total

27.6 ± 0.72 a

100.0

  1. Data are expressed as units per gram wet weight (U/gww), means ± SEM, for determinations on muscle preparations from n = 8 individual frogs. a Significantly different from the corresponding value in control muscle by the Student's t-test, P < 0.05; percentage data were evaluated after arcsine √ transformation of the raw data.
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BMC Biochemistry

ISSN: 1471-2091

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