Sequence alignment of the predicted Wzb protein from L. rhamnosus strain ATCC 9595 (GenPept Acc. No. AAW22448). Similar proteins from polysaccharide biosynthesis loci are as follows: AAG44708 is from Lactobacillus delbrueckii subsp. bulgaricus Lfi5 (EpsD); P96717 is from Bacillus subtilis 168 (YwqE/PtpZ); NP_053031 is from Lactococcus lactis subsp. cremoris B40 (EpsC); AAB49432 is from Staphylococcus aureus 8C (Cap8C); AAK61896 is from Streptococcus thermophilus Sfi39 (EpsB); AAC69525 is from Streptococcus pneumoniae 23F (Cps23fB). The four conserved motifs of the PHP domain (PF02811) are underlined, identical residues are indicated with an asterisk, while conserved residues and substitutions are indicated with dots. Mutations inserted in the wzb gene are numbered and give the following amino acid changes: 1: D3N, 2: H5A-H7A, 3: C6S, 4: H42A-H43A, 5: D64N, 6: R68A, 7: R69A, 8: C78S, 9: H197A-H198A (residue numbering corresponds to wild type Wzb amino acids).