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Figure 4 | BMC Biochemistry

Figure 4

From: Association of SAP130/SF3b-3 with Cullin-RING ubiquitin ligase complexes and its regulation by the COP9 signalosome

Figure 4

Interaction of SAP130 with HA-Cul1. (A) A diagram indicating the Cul1 truncation constructs and the interaction results. (B) HA-Cul1 and its truncations HA-Cul1N428, HA-Cul1C450 and HA-Cul1C280 were transiently expressed in HEK293 cells. The HA-IP samples were blotted with indicated antibodies. (C) HA-Cul1 and its truncations as indicated were transfected into Flag-SAP130 stable cells. Flag IP was performed and samples were probed with anti-HA antibody. (D) HeLa cells were transfected with HA-Cul1 or vector. Cell extracts were incubated with anti-CSN2 antibody or pre-immune serum for 15 min at room temperature and then blotted for Cul1 or HA. Arrowheads indicate endogenous Cul1 and the lines on the right indicate overexpressed HA-Cul1.

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BMC Biochemistry

ISSN: 1471-2091

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