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Figure 3 | BMC Biochemistry

Figure 3

From: Association of SAP130/SF3b-3 with Cullin-RING ubiquitin ligase complexes and its regulation by the COP9 signalosome

Figure 3

SAP130 forms tertiary complexes with CRL substrate adaptors and is associated with E3 activity. (A) HEK293 cells were transfected with HA-VHL or Flag-SAP130. Anti-HA and anti-Flag IP samples were blotted with indicated antibodies. An asterisk (*) indicates a non-specific band in Flag IP. (B) HA-SAP130 was expressed in HEK293 cells. Anti-HA IP samples were probed with indicated antibodies. (C) Flag immunocomplex isolated from HEK293 cells expressing empty vector, Flag-SAP130 or Flag-CPSF160 were incubated with N-terminal biotin-labeled ubiquitin, E1 and E2 (UbcH5b) as indicated. Polyubiquitination was determined by the detection of biotin-Ub.

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BMC Biochemistry

ISSN: 1471-2091

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