Table 4 Oligonucleotide primers used for the construction of plasmids. Primers were used in the amplification reactions to generate various DNA fragments or mutations constructs.
From: Role of the linker region in the expression of Rhizopus oryzae glucoamylase
Name | Nucleotide sequence* |
|---|---|
F-Ro GA | 5'-TTCGAATTC ATGCAATTATTCAATTTG-3' |
R-Ro GA | 5'-TTCGAATTC TTAAGCGGCAGGTGCACC-3' |
R-GAΔ168–604 | 5'-TTGAATTC TCAGTTACCAGTTGGGA-3' |
R-GAΔ132–604 | 5'-TTGAATTC TCATGTAGATACTTGGT-3' |
F-PS1 | 5'-CGTAGTTTTTCAAGTTCTTAG-3' |
R-PS1 | 5'-TCCTTACCTTCCAATAATTC-3' |
F-GAΔ132–167 | 5'-CAAGTATCTACATCTACAATCTCC-3' |
R-GAΔ132–167 | 5'-AGGAGATTGTAGATGTAGATACTTG-3' |
F-GAΔ26–131 | 5'-TTGCTTGTTTCTGCTTCCAAGCCCACTACT-3' |
R-GAΔ26–131 | 5'-AGTAGTGGGCTTGGAAGCAGAAACAAGCAA-3' |
F-GAΔ26–145 | 5'-AGAAGTAGAAGGGGCAGCAGAAACAAGCAA-3' |
R-GAΔ26–145 | 5'-TTGCTTGTTTCTGCTGCCCCTTCTACTTCT-3' |
F-GAΔ26–160 | 5'-TTGCTTGTTTCTGCTGCTACTTTCCCAACT-3' |
R-GAΔ26–160 | 5'-AGTTGGGAAAGTAGCAGCAGAAACAAGCAA-3' |
F-GAΔ26–167 | 5'-TTGCTTGTTTCTGCTTCTACAATCTCCTCA-3' |
R-GAΔ26–167 | 5'-TGAGGAGATTGTAGAAGCAGAAACAAGCAA-3' |
F-T165A | 5'-CCAGCT GGTAACTCTACAATCTCCTCA |
R-T165A | 5'-GGAGATTGTAGAGTTACCAGC TGG |
F-N167D | 5'-ACTGGTGAC TCTACAATCTCCTCATGGATT-3' |
R-N167D | 5'-GGAGATTGTAGAGTC ACCAGTTGGGAAAGT-3' |