Role of the linker region in the expression of Rhizopus oryzae glucoamylase

BMC Biochemistry

Table 2 Comparison between calculated and experimental molecular weights of GAs. The molecular masses were calculated from the deduced amino acid compositions using the ExPASy Molecular Biology Server. The experimental molecular masses were obtained from mass spectrometry or estimated from SDS-PAGE.

Protein	Calculated mass (kDa)	Apparent mass (kDa) (mass spectrometry/SDS-PAGE)
full-length GA	62.4	ND*/78
T165A	62.4	ND*/78
N167D	62.3	ND*/76
GAΔ168–604	15.2	20.7/27
GAΔ132–604	11.7	12.0/14
GAΔ26–131	50.7	ND*/69
GAΔ26–145	49.4	59.9 to 61.1^†/60
GAΔ26–160	47.9	55.5 to 56.6^‡/55

*ND, not determined. ^†GAΔ26–145 displayed multiple mass spectral peaks ranging from 59.9 to 61.1 kDa. ^‡GAΔ26–160 displayed multiple mass spectral peaks ranging from 55.5 to 56.6 kDa.

ISSN: 1471-2091