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Table 1 Amino acid sequences of the linker region from fungal GAs. Potential glycosylation sites in the linker sequences were predicted by the NetNGlyc and NetOGlyc programs. The predicted N-glycosylation sites are underlined and the putative O-glycosylation sites are labeled in bold.

From: Role of the linker region in the expression of Rhizopus oryzae glucoamylase

GA*

Position (aa)

Linker sequence

Ro GA

132–167

SKPTTTT ATTTTTT APSTSTTT RPSSS EPAT FPT GN

An GA

494–531

AT GGTTTT AT PT GS GS VTSTS KTT AT AS KTSTSTSSTS

Hr GA

500–504

N VTSS

Hg GA

490–508

S KQT PNPS AAPS PS PYPT A

Mc GA

129–177

SVTTTTTT APTTTTS GGSSTTT GGSTTT ATS VPT GVPSGFPT GN ST ISS

Nc GA

494–519

AT AT ATS FPANLT PASTT VT PPT QTG

  1. *GenBank or GenPept accession numbers are as follows: Ro, Rhizopus oryzae (from Simpson Biotech); An, Aspergillus niger (AAP04499); Hr, Hormoconis resinae (Q03045); Hg, Humicola grisea (AAA33386); Mc, Mucor circinelloides (AAN85206); and Nc, Neurospora crassa (P14804).