Figure 3

Purification and deglycosylation of recombinant Ro GAs. (A) Purified proteins (1 μg) obtained by cation-exchange chromatography were subjected to electrophoresis on a 15% SDS-PAGE and stained with Coomassie Brilliant Blue R-250. Lane 1, full-length GA; lane 2, GAΔ168–604; lane 3, GAΔ132–604; lane 4, GAΔ26–131; lane 5, GAΔ26–145 and lane 6, GAΔ26–160. (B) Protein samples were treated with PNGase F (250 U) and resolved by 10% SDS-PAGE. Symbols (+) and (-) indicate treatment with and without PNGase F, respectively. (C) Recombinant SBD was treated with Jack bean α-mannosidase and examined by 15% SDS-PAGE. Symbols (+) and (-) indicate treatment with and without Jack bean α-mannosidase, respectively.