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Table 1 Table showing IC50 values of different purine and allopurinol-based compounds for XOD inhibition.

From: Preferential inhibition of xanthine oxidase by 2-amino-6-hydroxy-8-mercaptopurine and 2-amino-6-purine thiol

  

(μM ± SEM)

Inhibitor

 

Hypoxanthine

Xanthine

6MP

ALLOPURINOL

0.91 ± 0.01

2.36 ± 0.03

1.92 ± 0.03

APT

7.28 ± 0.17

16.38 ± 0.21

2.57 ± 0.08

AHMP

5.08 ± 0.04

17.71 ± 0.29

0.54 ± 0.01

APP

6.79 ± 0.28

20.76 ± 0.84

12.86 ± 0.69

  1. The reaction mixture comprised of 1 ml of 0.2 M, sodium phosphate buffer pH 7.4 containing 10 μM of one of the substrates namely, hypoxanthine, xanthine or 6MP. The concentration range of allopurinol and APP were 0–3 μM and 0–100 μM, respectively for all three substrates. The concentration range of AHMP and APT were 0–100 μM and 0–50 μM, respectively for all three substrates. The reaction was initiated by the addition of 2.8 U/ml of bovine XOD.
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BMC Biochemistry

ISSN: 1471-2091

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