Figure 9From: Preferential inhibition of xanthine oxidase by 2-amino-6-hydroxy-8-mercaptopurine and 2-amino-6-purine thiolLineweaver-Burk plot of inhibition of XOD-mediated 6MP hydroxylation by AHMP. The reaction mixture comprised of 1 ml of 0.2 M, sodium phosphate buffer pH 7.4 containing varying concentrations of 6MP ranging from 5, 8, 10 and 20 μM. AHMP concentrations were [I] = 0 μM (●), [I] = 0.5 μM (○), [I] = 1.5 μM (▼). The reaction was initiated by the addition of 2.8 U/ml of bovine XOD.Back to article page