Figure 6

Lineweaver-Burk plot of inhibition of XOD-mediated xanthine hydroxylation by APT. The reaction mixture comprised of 1 ml of 0.2 M, sodium phosphate buffer pH 7.4 containing varying concentrations of xanthine ranging from 2, 5, 8, 10 and 20 μM. APT concentrations were [I] = 0 μM (●), [I] = 5 μM (○), [I] = 10 μM (▼), [I] = 15 μM (▽). The reaction was initiated by the addition of 2.8 U/ml of bovine XOD.