Figure 5From: Preferential inhibition of xanthine oxidase by 2-amino-6-hydroxy-8-mercaptopurine and 2-amino-6-purine thiolBi-substrate reaction of XOD-mediated hydroxylation of xanthine and 6MP in presence of AHMP. The reaction mixture comprised of 1 ml of 0.2 M, sodium phosphate buffer pH 7.4 containing 10 μM of both xanthine and 6MP. 2 μM AHMP was taken. The reaction was initiated by the addition of 2.8 U/ml of bovine XOD and observed for 20 min.Back to article page