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Figure 1 | BMC Biochemistry

Figure 1

From: Revalidation and rationale for high pKa values of unconjugated bilirubin

Figure 1

Comparison of aqueous:chloroform partition ratios of radioactivity (14C-PR, ), using 14C-UCB, with partition ratios of unlabeled UCB (PR diazo ). PRdiazo was calculated from the equation derived from diazo-assay of the two phases [3], plotted against pH of the aqueous buffer. Panel A – Log PR vs. pH. Heavy solid line shows the PRdiazo derived from computer modeling the diazo-based data [3]. Panel B – The same data, plotted as the difference, log PRdiazo – log 14C-PR. In each panel, the heavy dashed lines represent the 95% confidence limits of the log PRdiazo data, equal to 2 × 0.191, the [root mean sq. deviation]0.5 of the log PRdiazo data [3]. Only one of the 14C-PR data points (at pH 7.4) falls barely outside the 95% confidence limits for the PRdiazo data.

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