Mapping of possible prion protein self-interaction domains using peptide arrays

BMC Biochemistry

Table 1 Monoclonal antibody overview

antibody	epitope^a	position^b	blocking^C (μg/ml)	reference^d
100B3	KRPKP	26–30	~0,5	Thuring, 2005 [30]
SAF32	QPHGGGW ^e	54–92	25,0	Feraudet, 2005 [51]
9A2	WNK	102–104	4,0	Langeveld, 2006 [47]
6C2	HVAGAAA	114–120	20,0	this paper
6H4	DYEDRYYRE	147–155	1,0	Korth, 1997 [18]
94B4	HTVTTTTK	190–197	10,0	Thuring, 2004 [52]
M7	QQSYGQEP	n.a.	n.a.	Bakker, pers. comm.

^a amino acid sequence of the reported antibody epitope using peptide mapping techniques
^b position of the antibody epitope(s) in the ovine protein sequence
^c concentration at which the antibody completely blocked binding on the peptide-array
^d publication in which the epitope mapping results are reported
^e epitope of octarepeat (partially); occurs as 5 respectively 6 successive sequences in ovine and bovine PrP

ISSN: 1471-2091