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Figure 3 | BMC Biochemistry

Figure 3

From: p42MAPK-mediated phosphorylation of xEIAP/XLX in Xenopus cytostatic factor-arrested egg extracts

Figure 3

p42MAPK phosphorylates Ser235/251/254 of xEIAP/XLX. (A) Amino acid sequence of xEIAP/XLX residues 219–269. Indicated three Ser residues are changed to either Ala or Asp. (B) Phosphorylation of WT, Ser235Ala single mutant (1A), Ser251/254Ala double mutant (2A), and Ser235/251/254Ala triple mutant (3A). (C) Phosphorylation of WT, 3A, and Ser235/251/254Asp triple mutant (3D). In (B) and (C), MBP-Δ1 variants were incubated with CSF-arrested egg extracts (CSF) or p42MAPK (MAPK) in the presence of [γ-32P] ATP. After separation by SDS-PAGE, incorporated radioactivity was detected by image analyzer. Same amounts of substrates were also stained with Coomassie Brilliant Blue (Input). In (B), the phosphorylation intensities were calculated using NIH ImageJ software and indicated below.

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