A novel bifunctional N-acetylglutamate synthase-kinase from Xanthomonas campestris that is closely related to mammalian N-acetylglutamate synthase

Table 4 Dependence of the synthase and kinase activities on pH. Enzymatic activity is defined as the number of μmoles of product formed in one minute.

pH	Synthase Specific Activity	pH	Kinase Specific Activity
6.5	0.59 ± 0.01^a	4.5	3.46 ± 0.42^b
7.0	2.75 ± 0.02	5.0	4.77 ± 0.56
7.5	3.66 ± 0.10	5.5	6.15 ± 0.04
8.0	4.67 ± 0.64	6.0	7.02 ± 0.02
8.5	12.18 ± 1.42	7.0	6.59 ± 0.11
9.0	18.33 ± 1.94	7.5	6.55 ± 0.33
10.0	15.16 ± 1.73	8.0	3.99 ± 0.21

^anumbers represent averages of two measurements and associated standard errors ^bnumbers represent averages of three measurements and associated standard errors

ISSN: 1471-2091