Table 2 Substrate specificity of XcNAGS-K. The concentrations of L-glutamate and its alternatives were 50 mM. The concentrations of ATP, CTP and UTP were 20 mM. One unit of activity is defined as one μmole of product produced in one minute.
Substrate | Synthase Activity (units/mg of protein) | Kinase Activity (units/mg of protein) |
|---|---|---|
L-Glutamate | 31.5 ± 0.0a | - |
D-Glutamate | ndb | - |
L-Glutamine | nd | - |
L-Aspartate | nd | - |
ATP | -c | 4.84 ± 0.00 |
CTP | - | 0.11 ± 0.01 |
UTP | - | 0.07 ± 0.01 |