Figure 5From: A novel bifunctional N-acetylglutamate synthase-kinase from Xanthomonas campestris that is closely related to mammalian N-acetylglutamate synthaseDependence of the synthase reaction of XcNAGS-K on the concentration of AcCoA and glutamate. A. AcCoA concentration was varied between 0 and 4 mM while glutamate concentration was fixed at 50 mM. B. Glutamate concentration was varied between 0 and 50 mM while AcCoA was fixed at 2 mM. The assays were performed either with XcNAGS-K fused with a polyhistidine tag (black) or without affinity tag (gray).Back to article page