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Figure 5 | BMC Biochemistry

Figure 5

From: A novel bifunctional N-acetylglutamate synthase-kinase from Xanthomonas campestris that is closely related to mammalian N-acetylglutamate synthase

Figure 5

Dependence of the synthase reaction of XcNAGS-K on the concentration of AcCoA and glutamate. A. AcCoA concentration was varied between 0 and 4 mM while glutamate concentration was fixed at 50 mM. B. Glutamate concentration was varied between 0 and 50 mM while AcCoA was fixed at 2 mM. The assays were performed either with XcNAGS-K fused with a polyhistidine tag (black) or without affinity tag (gray).

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