Figure 2

Phylogenetic tree of NAGS protein sequences from bacteria, fungi, algae, plants and vertebrates, and fungal NAGK. The tree was generated by the neighbor joining method using Phylip 3.6. Numbers indicate bootstrap values from 1000 replicas. Bootstrap values equal to 1000 are not indicated in the figure due to spatial constraints. NAGS, NAGSK and NAGS/K proteins are shown in blue, red and purple typeface, respectively. Proteins from M. maris, O. alexandrii and P. bermudensis are shown in black typeface because their ability to catalyze synthase reaction, kinase reaction, or both has not been examined experimentally. NAGS, NAGK and NAGS-K from the following organisms were included in the phylogenetic analysis: human – Homo sapiens, mouse – Mus musculus, rat – Ratus norvegicus, cow – Bos torus, dog – Canis familiaris, frog – Xenopus tropicalis, zebrafish – Danio rerio, pufferfish – Fugu rubripes, freshwater pufferfish – Tetraodon nigroviridis, soy – Glycine max, corn – Zea mayis, tomato – Solanum lycopersicum, rice – Oryza sativa, Arabidopsis – Arabidopsis thaliana, S. cerevisiae – Saccharomyces cerevisiae, S. pombe – Schizosaccharomyces pombe, C. albicans – Candida albicans, N. crassa – Neurospora crassa, D. discoideum – Dictiostelium discoideum, X. campestris – Xanthomonas campestris, X. axonopodis – Xanthomonas axonopodis, X. fastidiosa – Xylella fastidiosa, M. maris – Maricaulis maris, O. alexandrii – Oceanicaulis alexandrii, P. bermudensis – Parvulalcula bermudensis, P. aeruginosa – Pseudomonas aeruginosa, P. syringiae – Pseudomonas syringiae, N. gonorrhoeae – Neisseria gonorrhoeae, S. typhimurium – Salmonella typhimurioum, E. coli – Escherichia coli.