Kinetic characterisation of arylamine N-acetyltransferase from Pseudomonas aeruginosa

BMC Biochemistry

Table 3 Determination of the half-life of the acetyl-enzyme intermediate.

Acetyl Donor	Rate of hydrolysis of acetyl donor (nM·s^-1)^a			k₃ (× 10^-3·s^-1)^b	t_1/2 (s)^c
	No enzyme	4 mM PANAT	8 mM PANAT
p-Nitrophenyl acetate	0.28 ± 0.01	12.2 ± 0.5	22.8 ± 0.4	2.83 ± 0.08	235 ± 8
AcCoA	ND^d	-	21.2 ± 3.9	2.66 ± 0.49	270 ± 49

^aThe rates of hydrolysis were determined spectrophotometrically by measuring the rate of production of p-nitrophenol or CoA, as described in the text.
^bk₃ values were determined according to equation 9 (Figure 3).
^ct_1/2 values were calculated according to equation 8 (Figure 3).
^dND, none detected.

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