Figure 8
From: Binding to DPF-motif by the POB1 EH domain is responsible for POB1-Eps15 interaction
Mutations in lysine residues distant from the binding pocket affect the interaction of POB1 with Eps15. Residues K312, W324, F344 and K351 in the POB1-EH domain were changed into Ala and the recombinant proteins were tested in an Eps15 pull-down assay from Hek293. Bound proteins were resolved by SDS-PAGE and analyzed by western-blotting using an anti-Eps15 antibody. In the lower panel, the GST fusions are visualized by Coomassie staining. Relative binding efficiencies represent the quantification of the Western blotting using the Image Quant Software.