Figure 6From: Binding to DPF-motif by the POB1 EH domain is responsible for POB1-Eps15 interactionThe DPF region of Eps15 binds to Eps15 in a pull-down experiment. 50 μg of the Eps15 recombinant proteins spanning amino acids 623–750 and comprising 10 DPF tripeptides, as described in Figure 3, were incubated with a cell extract from Hek293. Bound proteins were resolved by SDS-PAGE and analyzed by western-blotting using an anti-Eps15 antibody.Back to article page