Figure 3

The EH domain of POB1 binds to an 18 amino acids fragment comprising residues 623–640 and including three DPF. (A) Schematic representation of the C-terminal DPF-region of mouse Eps15, spanning amino acids 623–750 and comprising 10 DPF tripeptides. The recombinant proteins are progressive COOH-terminal deletions of 10 amino acids fused to the GST. The bars represent the protein fragments that are retained in the recombinant protein. The DPF motifs are indicated by asterisks. (B) Lysates of Hek 293Phoenix expressing Myc-POB1 were incubated with GST or equal amounts of the recombinant proteins bound to glutathione-sepharose beads. Adsorbed proteins were identified with anti-Myc antibody. In the lower panel the GST fusions are visualized with Coomassie staining.