Figure 2
From: Binding to DPF-motif by the POB1 EH domain is responsible for POB1-Eps15 interaction
The EH domain of POB1 binds to both NPF and DPF-containing peptides. Above: phage clones displaying different peptides were adsorbed to a plastic microtiter plate and incubated with the EH domain of POB1 fused to GST and GST alone as negative control. The bound domain was identified with an anti-GST antibody and a secondary antibody linked to alkaline phosphatase; wt is a phage not exposing any ectopic peptide. Below: sequences of the selected peptides are aligned with respect to the NPF and DPF motifs.