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Figure 2 | BMC Biochemistry

Figure 2

From: Downregulation of COP9 signalosome subunits differentially affects the CSN complex and target protein stability

Figure 2

The CRL component Rbx1 is destabilized in CSN knockdown cells. (a) CHX chase experiments were performed with siGFP, siCSN1, siCSN3 and siCSN5 cells. After the indicated time cell lysates were analyzed by Western blotting using the anti-Rbx1 antibody. The α6 proteasomal subunit was probed as loading control. (b) Densitometric analysis of the Rbx1 signals from (a). The densitometric signal at 0 h was put to 100% and the percentage of Rbx1 signals was plotted against the indicated time.

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