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Figure 3 | BMC Biochemistry

Figure 3

From: Metabolic signature of breast cancer cell line MCF-7: profiling of modified nucleosides via LC-IT MS coupling

Figure 3

Constant neutral loss (CNL) chromatograms of a MCF-7 cell culture supernatant sample. The different CNL amu values are characteristic for the various ribose structures of the analyzed metabolites, lost as a neutral moiety in the MS/MS fragmentation of the labile C-N-glycosidic bond between sugar and base. Numbers refer to metabolites listed in table 1. ψ (no characteristic neutral loss because of stable C-C-glycosidic bond) and IAA-R (not detectable in supernatants of MCF-7) are not shown.

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