Figure 4

Normalized amplitudes of SPR sensorgrams obtained by injection of recoverin forms into flow cells coated with different lipid mixtures. (A) Binding of myristoylated and nonmyristoylated forms of both WT recoverin (black bars) and Rc^2–190 (gray bars) were compared by employing a pure PC and a PC:PS (50:50) surface. Protein concentration was 30 μM. Estimated ratios WT/Rc^2–190 of binding amplitudes were 1.8 (myristoylated, PC), 2.2 (myristoylated, PC/PS), 0.9 (nonmyristoylated, PC) and 2.0 (nonmyristoylated, PC/PS). (B) Binding of myristoylated WT recoverin (black bars) and Rc^2–190 (gray bars) on a PC:PE (50:50) or a PC:PE:PS (40:40:20) surface yielding ratios of 3.0 and 2.3, respectively. Protein concentration was 5 μM. Data were collected from 2–6 experiments.