Figure 1

Binding of myristoylated WT recoverin to different lipids or lipid mixtures. Binding of recoverin to lipid mixtures was analyzed by an equilibrium centrifugation assay as described in the Methods section. The composition of the lipid mixtures was as follows: PE, 100% (w/w) phosphatidylethanolamine; PC, 100% (w/w) phosphatidylcholine; PE-PC, 50% (w/w) phosphatidylethanolamine, 50% (w/w) phosphatidylcholine; PE-PC-PS, 25% (w/w) phosphatidylethanolamine, 25% (w/w) phosphatidylcholine, 50% (w/w) phosphatidylserine; PS, 100% (w/w) phosphatidylserine; PE-PC-Ch, 25% (w/w) phosphatidylethanolamine, 25% (w/w) phosphatidylcholine, 50%(w/w) cholesterol; PE-PC-Ch-Ps, 20% (w/w) phosphatidylethanolamine, 20% (w/w) phosphatidylcholine, 50% (w/w) cholesterol, 10% (w/w) phosphatidylserine.