|
WT |
A1P |
A1c1A |
A12AP |
A1DAP |
A1I |
A1G |
---|
KD (M)a | 3 ± 2 × 10-10 | 9 ± 2 × 10-10 | 7 ± 2 × 10-10 | 4 ± 1 × 10-8 | 4 ± 2 × 10-8 | 1.6 ± 0.1 × 10-8 | 1.4 ± 0.4 × 10-6 |
ΔG (kcal/mol)b | -13.0 ± 0.4 | -12.3 ± 0.4 | -12.5 ± 0.2 | -10.1 ± 0.1 | -10.1 ± 0.3 | -10.6 ± 0.02 | -8.0 ± 0.1 |
ΔΔGc |  | 0.7 | 0.5 | 2.9 | 2.9 | 2.4 | 5.0 |
- aKD values are the average of at least three independent experiments. Standard deviations determined from the independent measurements are reported. bΔG is the free energy of association of the complex. cΔΔG is the difference between the binding energies of the wild type U1A protein for SL2 RNA containing the indicated base substitution and wild type SL2 RNA.