Skip to main content

Table 1 Stability of complexes of wild type U1A protein with SL2 RNA sequences containing A1 modifications.

From: Recognition of essential purines by the U1A protein

 

WT

A1P

A1c1A

A12AP

A1DAP

A1I

A1G

KD (M)a

3 ± 2 × 10-10

9 ± 2 × 10-10

7 ± 2 × 10-10

4 ± 1 × 10-8

4 ± 2 × 10-8

1.6 ± 0.1 × 10-8

1.4 ± 0.4 × 10-6

ΔG (kcal/mol)b

-13.0 ± 0.4

-12.3 ± 0.4

-12.5 ± 0.2

-10.1 ± 0.1

-10.1 ± 0.3

-10.6 ± 0.02

-8.0 ± 0.1

ΔΔGc

 

0.7

0.5

2.9

2.9

2.4

5.0

  1. aKD values are the average of at least three independent experiments. Standard deviations determined from the independent measurements are reported. bΔG is the free energy of association of the complex. cΔΔG is the difference between the binding energies of the wild type U1A protein for SL2 RNA containing the indicated base substitution and wild type SL2 RNA.