Steps | 1 | 2 | 3 | 4 |
---|
Process | %Release (with G6P) | Blocking (with DCCD) | %Release (with KSCN) | Rebinding |
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Rat | 81 ± 3 | Yes | 30 ± 2.1 | N.D. |
Bovine | 38 ± 2.7 | Yes | 19 ± 1.7 | N.D. |
Human | 48.5 ± 3.5 | Yes | 17.5 ± 2.5 | N.D. |
- Mitochondria from three species were subjected to HK release by G6P (2 mM) in the first step. Then, the exposed binding sites of G6P – treated mitochondria were blocked by DCCD (0.01 mM). At the third step, the remaining HK activity on this DCCD-treated mitochondria was released with 45 mM KSCN. Finally the depleted (both sites) blocked (G6P-sensitive sites) mitochondria were used for the rebinding process, according to details described under Methods. N.D.; not detectable