A study on the two binding sites of hexokinase on brain mitochondria

BMC Biochemistry

Table 1 Solubilization of mitochondrially- bound hexokinase of rat, bovine and human brain tissues' mitochondria by sequential treatment with G6P and KSCN

	Treatment	Rat	Bovine	human
% Release	First, with G6P	80 ± 2.1	46 ± 0.8	55 ± 1.8
	Second, with KSCN	10 ± 0.8	9.2 ± 0.75	7.7 ± 1.85
	First, with KSCN	33.4 ± 0.8	8.8 ± 0.3	5.1 ± 0.075
	Second, with G6P	51 ± 7.6	44.2 ± 4.3	53 ± 0.95

Solubilization of hexokinase by G6P (2 mM) and KSCN (45 mM) was determined as noted under Methods. The remaining activity of HK on G6P treated mitochondria (e.g. for rat; 20%), was subject to release by KSCN. Ten percent of the enzyme activity could be released in this process. Total activity which could be released in the two steps, never exceed 100%. Values shown are means ± SE of at least 3 determinations.

ISSN: 1471-2091