Figure 4

mRNA expression levels of Macaca fascicularis 17β-HSD12 measured by Q_RTPCR. mRNA expression levels were quantified in the adrenal gland, ovary, mammary gland, endometrium, myometrium, cerebral cortex, cerebellum, liver, pancreas, heart, testis, prostate, kidney, skin, muscle and spleen by Quantitative RTPCR (Q_RTPCR). The reaction was performed using the amount of cDNA corresponding to 30 ng of initial total RNA following the manufacturer's protocol. All sample were run in duplicates and quantification of each target gene expression was done two or three times. Results are expressed as mean ± SEM.