Figure 2
Determination of the enzymatic activities of Macaca fascicularis 17β-HSD12 in intact transfected 293 cells in culture. Cells stably transfected with mf 17β-HSD12 were seeded into 6-well plates at a density of 5 × 105 cells/well. 0.1 μM of [14C]-labeled El, E2, 4-dione, and T were added to freshly changed culture medium. Non transfected HEK-293 cells were used as controls. After 20 h of incubation, the media were collected and extracted. The data are expressed as a mean ± SEM of triplicate measurements. ** indicates significantly different from non transfected HEK-293 cells at p < 0.01.