Figure 3From: The subunit composition of human extracellular superoxide dismutase (EC-SOD) regulate enzymatic activitySchematic representation of disulfide-linked tryptic peptides. A representation of human EC-SOD is depicted with the cysteine residues involved in intramolecular disulfide bonding indicated and tryptic cleavage sites generating cysteine-containing peptides shown by arrows. Following cleavage by trypsin, aEC-SOD is represented by a disulfide-linked triple peptide and iEC-SOD represented by a disulfide-linked double peptide. These two structures can be separated by RP-HPLC and can thus be used to detect the presence of the two folding variants.Back to article page