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Figure 7 | BMC Biochemistry

Figure 7

From: R7-binding protein targets the G protein β5/R7-regulator of G protein signaling complex to lipid rafts in neuronal cells and brain

Figure 7

Distribution of endogenous Gβ5/RGS7/R7BP among detergent-resistant membrane (DRM) and non-DRM fractions of mouse brain membranes. A. Analysis of endogenous marker proteins in DRM and non-DRM fractions from mouse brain. Equal volumes of gradient fractions were analyzed by immunoblotting for flotillin-1, as marker for DRM, and the transferrin receptor (TfR) as a non-DRM marker. B. Sucrose density gradient analysis of endogenous Gβ5, RGS7, and R7BP synaptosomal membrane proteins in mouse brain. A detergent extract of mouse brain synaptosomes was subjected to a sucrose density gradient fractionation. Equal aliquots from all eleven fractions were subjected to SDS-PAGE followed by immunoblotting for the endogenous Gβ5, RGS7, and R7BP (using antibody TRS) proteins as indicated. Immunoblots of the sucrose density gradients shown were analyzed by densitometry and the distribution of the indicated immunoreactive bands between DRM and non-DRM fractions among (as % of total immunoreactivity with that antibody) is shown as a histogram to the right of the corresponding immunoblot, with error bars representing the S.E.M.

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BMC Biochemistry

ISSN: 1471-2091

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