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Figure 4 | BMC Biochemistry

Figure 4

From: R7-binding protein targets the G protein β5/R7-regulator of G protein signaling complex to lipid rafts in neuronal cells and brain

Figure 4

Effect of R7BP on the distribution of endogenous Gβ5/RGS7 proteins into Triton X-100 soluble and insoluble fractions in PC12 cells. A. PC12 cells transfected with vector only (left), wild type (wt) HA-R7BP (middle) or HA-R7BP SS (right) were harvested, lysed and fractionated into Triton X-100 soluble and insoluble fractions. Proteins in each fraction were probed for HA-R7BP, Gβ5 and RGS7 and α-tubulin by immunoblotting with specific antibodies as shown. The data shown are representative of three experiments performed with similar results. B. The percentage of total transfected HA-R7BP or endogenous Gβ5 or RGS7 protein in the Triton X-100 soluble (Sol) or insoluble (Insol) fractions was determined by densitometry for the different transfection conditions as shown, with error bars representing the S.E.M.

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BMC Biochemistry

ISSN: 1471-2091

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