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Figure 3 | BMC Biochemistry

Figure 3

From: R7-binding protein targets the G protein β5/R7-regulator of G protein signaling complex to lipid rafts in neuronal cells and brain

Figure 3

Subcellular distribution of wild-type and mutant R7BP in PC12 cells. A. Cytoplasmic and nuclear distribution of transfected wild type R7BP or non-palmitoylated mutant R7BP in PC12 cells. Cytoplasmic and nuclear fractions were prepared from PC12 cells transfected with the cDNAs indicated on the left. Immunoblots to monitor HA-R7BP expression, α-tubulin expression as a marker for the cytosolic fraction, and p84/N5 expression as marker for the nuclear fraction are shown. B. Immunocytofluorescent localization of R7BP in PC12 cells. PC12 cells transfected with vector (panels in left column), wild type (wt) HA-R7BP (middle column) or HA-R7BP SS (right column), were subjected to the immunostaining using anti-HA primary antibody and fluorescein isothiocyanate (FITC)-conjugated donkey anti-mouse IgG antibody (green signal) as a secondary antibody. The Hoechst 33342 nuclear dye (blue signal) was used to counterstain transfected PC12 cells. The single or combined (merge) fluorescent signal was recorded by laser confocal microscopy. The results shown are representative of three total experiments performed with similar findings.

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BMC Biochemistry

ISSN: 1471-2091

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